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相关概念视频

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...

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相关实验视频

Updated: Jun 19, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

奇比 (Chibby) 是Wnt/Wingless通路的一个核β-catenin相关的对手.

Ken-Ichi Takemaru1, Shinji Yamaguchi, Young Sik Lee

  • 1Howard Hughes Medical Institute, Room K536C Health Sciences Building, Campus Box 357750, Department of Pharmacology, and Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195, USA.

Nature
|April 25, 2003
PubMed
概括

一种新发现的蛋白质Chibby抑制了β-catenin活性,这是许多癌症的关键驱动因素. 这一发现为Wnt/Wingless途径和潜在的癌症疗法提供了新的见解.

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The Soft Agar Colony Formation Assay
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The Soft Agar Colony Formation Assay

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

相关实验视频

Last Updated: Jun 19, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 18, 2014

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

科学领域:

  • 分子生物学分子生物学
  • 发育生物学 发展生物学
  • 癌症研究 癌症研究

背景情况:

  • 异常激活β-catenin是人类癌症发展的一个关键因素.
  • 在正规的Wnt/Wingless通路中,β-catenin充当协活性剂,与Tcf/Lef转录因子相互作用.

研究的目的:

  • 为了确定与β-catenin相互作用的新型蛋白质.
  • 阐明Chibby在β-catenin介导的转录激活和Wnt/Wingless通路中的作用.

主要方法:

  • 蛋白质相互作用查以确定Chibby.
  • 哺乳动物细胞培养试验研究了Chibby对β-catenin的抑制作用.
  • 在Drosophila中的RNA干扰,以评估Chibby的功能 in vivo.
  • 经验实验以确定Chibby在Wnt/Wingless路径中的位置.

主要成果:

  • 奇比直接与β-catenin的C端区域相互作用.
  • 奇比通过与Lef-1竞争以在哺乳动物细胞中与β-catenin结合来抑制β-catenin介导的转录激活.
  • Chibby在Drosophila的RNA干扰无翼的副本获得功能,导致细分极性缺陷和目标基因的过度表达.
  • 经验分析将奇比置于无翼的下游和鸟的上游.

结论:

  • 奇比是一种保存的核蛋白,可以负面调节β-catenin活性.
  • 奇比在Wnt/Wingless信号通路中起着至关重要的作用,作为一个新的对手.
  • 奇比的发现为了解和潜在地针对β-catenin驱动的癌症提供了新的途径.