人类大动脉的化与骨质细胞表型有关
Nalini M Rajamannan1, Malayannan Subramaniam, David Rickard
1Division of Cardiology, Northwestern University Feinberg School of Medicine, Northwestern University, 201 East Huron St, Galter Suite 10-240, Chicago, Ill 60611, USA. n.rajamannan@northwestern.edu
Circulation
|April 30, 2003
概括
大动脉结石化是一种常见的心血管疾病,涉及到类似于骨形成的活跃,受调节的过程. 这一过程与骨质母细胞类的表型有关,这表明一种受调节的机制而不是随机变性.
科学领域:
- 心血管生物学 心血管生物学
- 骨的新陈代谢 骨的新陈代谢
- 生物矿物化 生物矿物化
背景情况:
- 动脉狭窄是一种普遍的心血管疾病.
- 大动脉结石化的潜在机制仍然不完全理解.
- 一个假设表明,类似于由骨质样细胞介导的骨骨形成.
研究的目的:
- 为了研究大动脉结石化的机制.
- 为了确定大动脉的化是否涉及骨质细胞类型的表型.
- 为了比较化与正常人类大动脉的基因和蛋白质表达.
主要方法:
- 使用微射线图和微CT分析人类大动脉 (n=22化,n=20正常) 的分析.
- 组织学染色 (von Kossa,Goldner's) 用于识别矿物化.
- 电子显微镜和能量分散光谱用于超结构和元素分析.
- 反转录酶-聚合酶连锁反应 (RT-PCR) 用于骨质细胞标记物 (骨质,骨质蛋白,骨质,性酸酶,Cbfa1).
主要成果:
- 微射线和微CT证实了门的化.
- 组织学染色和电子显微镜确定了酸和CaPO4的组成.
- 通过RT-PCR检测,在化门中,骨质蛋白,骨质素和Cbfa1的mRNA水平增加.
- 在患病的门中,性酸酶显示蛋白质表达增加,但mRNA没有增加.
结论:
- 大动脉的化是一个活跃的,受调节的过程.
- 这个过程与骨质母细胞样细胞表型有关.
- 这些发现支持在大动脉结石化过程中存在类似骨形成的机制.
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