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相关概念视频

Protein Folding01:22

Protein Folding

Overview
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...

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相关实验视频

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

在高分子量蛋白质中Ile delta 1甲基的侧链分配:对46ns翻转分子的应用.

Vitali Tugarinov1, Lewis E Kay

  • 1Protein Engineering Network Centre of Excellence and the Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5S 1A8.

Journal of the American Chemical Society
|May 8, 2003
PubMed
概括

一个新的3DNMR脉冲方案, (H) C CA) NH-COSY,改善了大蛋白质中异黄素 (Ile) 甲基化学转移的分配. 与甲基分配的TOCSY方案相比,这种方法提高了灵敏度和分辨率.

科学领域:

  • 结构生物学 结构生物学
  • 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学
  • 蛋白质化学 蛋白质化学

背景情况:

  • 在异黄素 (Ile) 残留物中分配甲基的化学转移对于蛋白质结构的确定至关重要.
  • 传统的TOCSY (总相关谱) 方案与分支氨基酸和大型蛋白质进行斗争,导致灵敏度和光谱质量降低.
  • 高分子量蛋白 (40-50 ns的相关时间) 对现有的NMR分配方法构成挑战.

研究的目的:

  • 开发一种灵敏的3D核磁共振脉冲方案,用于分配 (13) C ((delta) ((1) 大型,无化,甲基质子蛋白中的化学转移.
  • 为了克服TOCSY方案在分支氨基酸侧链分配中的局限性.
  • 在具有挑战性的蛋白质系统中提高用于甲基组分配的光谱灵敏度和分辨率.

主要方法:

  • 开发和应用一个新的3DNMR脉冲方案: (H) C ((CA) NH-COSY.
  • 使用基于COSY的转移步骤来规避TOCSY的局限性.
  • 重定位不必要的 (13) C-(13) C 标量合器,以提高传输效率.
  • 在5°C的温度下对甲基原型马尔结合蛋白 (MBP) /β-环氧化复合物进行测试.

主要成果:

  • 在MBP复合体中的19个Ile残留物中的18个中成功分配了13C(delta 1) 化学转移.

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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  • 与基于TOCSY的类似方法相比,显著提高了灵敏度和分辨率.
  • 验证了 (H) C ((CA) NH-COSY方案对大型甲基质原蛋白的疗效.

    • 结论:

    • (H) C ((CA) NH-COSY脉冲方案是一种灵敏而有效的工具,用于分配 (13) C ((delta 1) 的化学变化.
    • 这种方法在大型,无化蛋白质中的甲基分配方面比TOCSY提供了更高的性能.
    • 开发的核磁共振技术促进了复杂蛋白质系统的结构生物学研究.