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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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相关实验视频

Updated: Jul 5, 2026

DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

对化学线索的打印:在鱼的家庭流选择的基础.

A T Scholz, R M Horrall, J C Cooper

    Science (New York, N.Y.)
    |June 18, 1976
    PubMed
    概括

    在发育过程中暴露于特定化学物质的科霍鱼在几年后可以重新找到那些相同的化学气味. 这项研究表明,鱼使用印记和化学线索来定位.

    科学领域:

    • 鱼类学 鱼类学 鱼类学
    • 动物行为 动物行为
    • 感官生态学 感官生态学

    背景情况:

    • 鱼的寻找行为对于维持种群至关重要.
    • 鱼追踪背后的感觉机制尚未完全被理解.
    • 在生命早期阶段的化学印记可能在指导中发挥作用.

    研究的目的:

    • 调查coho鱼 (Oncorhynchus kisutch) 在早期发育过程中是否会在化学线索上留下印记.
    • 为了确定这些印制的化学线索是否用于在产卵迁移期间的指导.
    • 评估特定化学物质 - - 吗啡和醇在鱼养中所起的作用.

    主要方法:

    • 年轻的科霍鱼在1.5个月内被暴露在摩尔福林或乙醇中.
    • 鱼类被释放到密歇根湖,并在18个月后监测它们的回归迁徙.
    • 量化了暴露鱼类的数量,这些鱼类返回了带有摩尔福林或乙醇气味的溪流.
    • 控制站点也被监测,以评估站点的真实性.

    主要成果:

    • 显著的大部分暴露于吗啡的鱼返回了带有吗啡气味的河流.
    • 大多数暴露于乙醇的鱼在乙醇气味的河流中被重新捕获.
    • 鱼类显然更喜欢与他们早期的化学物质暴露相匹配的溪流.

    更多相关视频

    A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying
    04:52

    A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

    Published on: June 1, 2018

    Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
    06:00

    Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

    Published on: March 17, 2023

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    Last Updated: Jul 5, 2026

    DNA-based Fish Species Identification Protocol
    09:15

    DNA-based Fish Species Identification Protocol

    Published on: April 28, 2010

    A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying
    04:52

    A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

    Published on: June 1, 2018

    Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
    06:00

    Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development

    Published on: March 17, 2023

    结论:

    • 科霍鱼表现出对其早期生命阶段经历的化学线索的印象.
    • 这些印制的化学线索被有效地用于在产卵期间的导航指导.
    • 化学印记是太平洋鱼回归行为的一个重要因素.