Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Adrenergic Antagonists: ɑ and β-Receptor Blockers01:31

Adrenergic Antagonists: ɑ and β-Receptor Blockers

Third-generation β-blockers, such as labetalol and carvedilol, represent a significant advancement in managing cardiovascular conditions. Unlike conventional β-blockers, which can induce peripheral vasoconstriction, third-generation drugs block α1 adrenoceptors. This promotes vasodilation through several mechanisms, such as increased nitric oxide production, inhibition of calcium ion entry, opening of potassium ion channels, and antioxidant action. Labetalol, for instance, is clinically...
Antihypertensive Drugs: Action of β1 Blockers01:17

Antihypertensive Drugs: Action of β1 Blockers

β1-receptors are primarily located in the heart and kidneys. In cardiac myocytes, these receptors interact with neurotransmitters released by the sympathetic nervous system during heightened activity or danger. As a result, β1-receptors get activated, initiating a series of biochemical processes. Excessive activation of beta receptors due to chronic stress can abnormally increase heart rate and contractility, resulting in high blood pressure or hypertension. To counteract this, β1-blockers...
Antihypertensive Drugs: Types of β-Blockers01:28

Antihypertensive Drugs: Types of β-Blockers

β receptors are classified into three subclasses: β1, β2, and β3. β1 receptors are primarily located in the heart and kidneys. When they get activated, they increase heart rate, contractility, and renin release. This process enhances blood pressure and aids in stress management. In contrast, β2 receptors are situated mainly in the lungs, blood vessels, and skeletal muscles. Upon activation, they trigger smooth muscle relaxation, causing bronchodilation and vasodilation. This widens airways and...
Antihypertensive Drugs: Vasodilators01:23

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
Antianginal Drugs: Nitrates and β-Blockers01:16

Antianginal Drugs: Nitrates and β-Blockers

In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart.
Organic nitrates,  such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Triglyceride/HDL-cholesterol ratio as a predictor for treatment-related severe hypertriglyceridemia in children with lymphoid malignancies.

Scientific reports·2026
Same author

Development of a novel protocol for processing fluorescent microspheres used in quantifying tissue perfusion.

Sensing and Bio-Sensing Research·2026
Same author

Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)-Enriched Extracellular Vesicles That Promote Angiogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Synchrotron beamline setup enabling quasi-simultaneous PXRD and XANES measurements: case study of Fischer-Tropsch catalyst reduction at 60 bar.

Journal of synchrotron radiation·2026
Same author

Red-complex bacteria: immunological background leading to the development of head and neck cancers.

Frontiers in immunology·2026
Same author

Beyond fluorodeoxyglucose: High-definition macrophage imaging to predict heart failure.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2026

相关实验视频

Updated: May 12, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

第三代β抑制剂通过ATP排泄刺激内皮细胞的氧化释放:一种新的抗高血压作用机制.

Leszek Kalinowski1, Lawrence W Dobrucki, Miroslawa Szczepanska-Konkel

  • 1Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio 45701, USA.

Circulation
|May 14, 2003
PubMed
概括

尼比沃洛和卡维迪洛通过刺激氧化 (NO) 的释放来促进血管放松. 这通过细胞外腺三酸盐 (ATP) 和微血管内皮细胞中的P2Y-纯受体激活发生.

更多相关视频

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
07:15

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

相关实验视频

Last Updated: May 12, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
07:15

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

科学领域:

  • 药理学 药理学是指药理学的学科.
  • 心血管研究研究心血管研究
  • 分子生物学分子生物学

背景情况:

  • 尼比沃洛和卡维迪洛是第三代β阻塞剂,具有独特的血管扩张特性.
  • 它们对微循环效应的机制,与经典β抑制剂不同,仍然不清楚.
  • 细胞外ATP是通过P2-purinoceptors在细胞功能中的关键信号分子.

研究的目的:

  • 调查nebivolol和carvedilol是否可以刺激微血管内皮细胞释放氧化 (NO).
  • 确定细胞外腺三酸盐 (ATP) 在这个过程中的作用.
  • 为了阐明P2-purinoceptors在β阻断剂诱导的血管扩张中的参与.

主要方法:

  • 测量囊血管收缩和放松.
  • 从单个质内皮细胞 (GEC) 中量化生物活性NO的释放.
  • 评估细胞外ATP水平并阻断P2Y-纯受体和ATP释放.

主要成果:

  • 尼比沃和卡维迪刺激了ATP和NO从GEC中以度依赖的方式释放.
  • 抑制ATP释放或P2Y-purinoceptors取消了β阻断剂诱导的血管松和NO释放.
  • 尼比沃洛和卡维迪洛的NO释放水平是可比的.

结论:

  • 尼比沃洛和卡维迪洛诱导脏球微血管放松.
  • 这种放松是由ATP流量介导的.
  • 该过程涉及刺激来自GECs的P2Y-purinoceptor依赖NO释放.