在心肌梗塞后重塑过程中对矩阵金属蛋白酶的特定区域和类型的诱导
Eric M Wilson1, Sina L Moainie, Julia M Baskin
1Medical University of South Carolina, Charleston, SC 29425, USA.
Circulation
|May 29, 2003
概括
在心肌梗塞 (MI) 后,矩阵金属蛋白酶 (MMP) 显示出特定区域的变化,影响心脏重塑. 针对这些MMP及其抑制剂 (TIMPs) 可能为后心脏病恢复提供新的治疗策略.
科学领域:
- 心血管研究研究心血管研究
- 生物化学 生物化学
- 病理生理学 病理生理学
背景情况:
- 矩阵金属蛋白酶 (MMPs) 在心肌梗塞 (MI) 后的不良心脏重塑中涉及.
- 在MI后的心肌中,MMPs的精确空间和特定类型的分布以前是未知的.
研究的目的:
- 调查心肌梗塞后心肌内MMP及其抑制剂 (TIMPs) 的区域和类型特定变化.
- 为了将这些分子变化与区域心脏几何学的变化相关联.
主要方法:
- 声微观测法用于测量在7个不同的心肌区域区域的区域细分长度变化,在绵羊后MI.
- 免疫洗量化了各种MMP和TIMP在偏远,过渡和MI地区的相对丰度.
主要成果:
- 在所有地区都观察到细分长度的显著增加,在MI区域最明显.
- 发现了MMP表达的不同模式:MMP-1和MMP-9在过渡和MI区域下降,而MMP-13,MMP-8和MT1-MMP在过渡和MI区域显著增加.
- 过渡区的TIMP丰度下降,在MI区变得无法检测,这表明MMP抑制的丧失.
结论:
- 心脏病发作后心脏几何变化与MMP水平的特定变化有关.
- 区域特异性的MMPs诱导,加上TIMPs的下降,表明MI后心肌中抑制控制的丧失.
- 针对MMP和TIMP的区域不平衡,为管理MI后不良改造提供了潜在的治疗途径.
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