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人类动脉样硬化斑块中的中性粒细胞弹性酶:由巨细胞产生
Clare M Dollery1, Caroline A Owen, Galina K Sukhova
1Leducq Center for Cardiovascular Research, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave, EBRC 307, Boston, Mass 02115, USA.
Circulation
|May 29, 2003
概括
中性粒细胞弹性酶 (NE),一种通常在中性粒细胞中发现的酶,存在于动脉样硬化斑块中,由巨细胞产生. 这表明NE在血管矩阵分解和动脉疾病中发挥了新的作用.
科学领域:
- 血管生物学 血管生物学
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 细胞外基质 (ECM) 代谢驱动健康和疾病中的血管重塑.
- 血清蛋白酶在动脉生成过程中血管ECM降解中的作用基本上是未知的.
- 人类中性粒细胞弹性酶 (NE),通常来自中性粒细胞,在与瘤相关的细胞中发现.
研究的目的:
- 研究中性粒细胞弹性酶 (NE) 在血管细胞外基质 (ECM) 降解中的作用.
- 确定动脉样硬化斑块内NE的存在和来源.
主要方法:
- 对人类动脉斑块 (纤维状和动脉瘤状) 和正常动脉进行组织学分析.
- 免疫组织化学检测NE和巨细胞.
- 在现场杂交以识别NE mRNA.
- 用单细胞,巨细胞和内皮细胞进行细胞培养实验,以评估NE的产生.
主要成果:
- 中性粒细胞弹性酶 (NE) 检测到纤维状和动脉瘤状斑块中,特别是在易受损伤的斑块中富含巨细胞的区域.
- 巨细胞和NE在易受伤害的斑块中结合在一起.
- 在富含巨细胞的区域发现了NE mRNA,这表明本地生产.
- 单细胞,巨细胞和内皮细胞在体外产生活性NE及其mRNA.
- 单细胞NE的产生是构成性的,但是在CD40连接体刺激后释放出来的.
结论:
- 中性粒细胞弹性酶 (NE) 在由巨细胞介导的矩阵降解中发挥着新的作用.
- 这种巨细胞衍生的NE对于血管重塑至关重要.
- NE有助于动脉疾病的发病,如动脉样硬化.
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