通过GLABRA2调节脂信号,以形成根毛发的模式
Yohei Ohashi1, Atsuhiro Oka, Renato Rodrigues-Pousada
1Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
概括
家庭盒基因GLABRA2 (GL2) 通过准光酶Dzeta1 (AtPLDzeta1) 基因来调节Arabidopsis的根毛发模式. 这种相互作用调节脂信号传递,影响根毛发的发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 细胞信号传递 细胞信号传递
背景情况:
- 在Arabidopsis thaliana中,根毛发育是一个由转录因子网络调节的复杂过程.
- 家庭盒基因GLABRA2 (GL2) 是这个网络中的关键下游调节器.
- 了解GL2的精确作用是解读根毛发模式机制的关键.
研究的目的:
- 研究GLABRA2 (GL2) 基因在阿拉比多普西斯根毛发发育中的下游目标和调控机制.
- 通过GL2.2直接调节的特定基因的识别.
- 阐明脂信号在GL2介导的根毛形成中的作用.
主要方法:
- 将GLABRA2 (GL2) 的基因改造包括一个交换激活功能.
- 在经过修改的Arabidopsis表皮细胞中分析异胎性根毛发的形成.
- 使用分子技术识别和验证GL2的直接基因标.
- 诱导式表达系统用于研究已识别的目标基因的功能.
主要成果:
- 一种经过修改的GL2蛋白诱导了表皮细胞中的宫外根毛或根毛状结构.
- 脂酶Djeta1基因 (AtPLDzeta1) 被确定为GL2.2的直接转录标.
- 诱导AtPLDzeta1的表达足以促进宫外根毛发的发育.
结论:
- GLABRA2 (GL2) 通过直接调节AtPLDzeta1.1.的表达来控制根毛发的生长.
- 根毛形成的GL2介导调节涉及脂信号通路的调节.
- AtPLDzeta1是GL2调节电路中的一个关键组件,用于根毛发模式.
更多相关视频
11:29Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
Published on: April 5, 2016
05:23Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
Published on: May 20, 2022
相关概念视频
Protein Glycosylation
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Protein Folding Quality Check in the RER
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Accessory Structures of the Skin: Hair and Hair Follicles
Hair and hair follicles are integral components of the integumentary system. Hair is a filamentous structure composed mainly of a protein called keratin. It is found on the surface of the skin throughout the body, except for areas such as the palms of the hands and soles of the feet.
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
