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相关概念视频

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
The DNA Helix01:16

The DNA Helix

Overview
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jul 6, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

对DNA单链和DNA:RNA混合体的NMR研究,有或没有C5的1-propynylation的寡头胺基因.

Brent M Znosko1, Thomas W Barnes, Thomas R Krugh

  • 1Department of Chemistry, University of Rochester, New York 14627-0216, USA.

Journal of the American Chemical Society
|June 6, 2003
PubMed
概括

通过增加单链预组织和有利的双链相互作用,DNA的propynylation增强了DNA:RNA混合稳定性. 这种修改为基基组提供了体积排除和增强的堆叠.

科学领域:

  • 化学生物学 化学生物学
  • 核酸化学的核酸化学
  • 结构生物学 结构生物学

背景情况:

  • 在DNA中的金字素在C5的1-propynylation可以显著提高DNA:RNA混合稳定性.
  • 之前的研究表明,每次替代在37摄氏度时,稳定性增加超过1 kcal/mol.
  • 了解propynylation的结构后果对于其在核酸稳定中的应用至关重要.

研究的目的:

  • 为了研究DNA寡核酸中的1-propynylation的结构后果.
  • 阐明基化DNA:RNA混合物的增强稳定性的结构基础.
  • 为了比较propynylated和non-propynylatedDNA:RNA复合体的结构动态和相互作用.

主要方法:

  • 采用二维NMR光谱学研究各种寡核酸的结构.
  • 内部残留核Overhauser效应光谱学 (NOESY) 用于探测单链预组织.
  • 确定DNA:RNA复合体的NMR结构,并对全球和本地结构特征进行分析.

主要成果:

  • 单链DNA (ssODN) 的propynylation表明了预组织,由特定的NOESY交叉峰表示.
  • 基化和非基化DNA:RNA复合体的全球结构几乎相同.

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

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  • 一个部分propynylated复合体 (sPrODN1:RNA) 的5'端表现出比一个完全propynylated复合体 (PrODN:RNA) 更大的动态.
  • 在基复合体中,基组堆叠在5'-基上,并延伸到主要槽中.

    • 结论:

    • 增强的稳定性propynylatedDNA:RNA杂交物是归因于单链预组织和改变双链相互作用.
    • 基组通过体积排除,增强基堆叠和潜在的修改溶解来促进稳定性.
    • 化为生物技术应用提供了一个调节核酸结构和稳定性的策略.