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菌素V行走手在手:单个光体成像与1.5纳米定位定位

Ahmet Yildiz¹, Joseph N Forkey, Sean A McKinney

¹Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, IL 61801, USA.

Science (New York, N.Y.)

|June 7, 2003

概括

肌V,一个分子电机,沿着动丝移动,以一种手对手的方式. 它的步骤大小,精确地测量,证实了这种交替运动,而不是一个英寸模型.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞力学细胞力学

背景情况:

氨酸V是一种关键的二维分子电机,负责沿着活性纤维的细胞内运输.
了解肌肉蛋白V的过程性运动的精确机制对于理解细胞功能至关重要.

研究的目的:

为了阐明myosin V在行为线丝上的运动过程中的步骤大小动态.
为了区分 proposed 拟议的myosin V运动模型,特别是手对手与英寸模型之间的区别.

主要方法:

菌素V被标记为单个光体在光链域内的各种位置.
用高精度 (<1.5 nm标准偏差) 和时间分辨率 (0.5 秒) 测量了步骤大小.
长时间的观察时间 (分钟) 用于捕捉多个步骤.

主要成果:

观察到myosin V的步骤大小在37 + 2x nm和37 - 2x nm之间交替.
参数"x"表示光体与两个髓素头之间的中间点之间沿运动方向的距离.
这些精确的测量提供了关于分步机制的定量数据.

结论:

观察到的交替的步骤大小强烈支持一个手对手模型的肌V运动.
这些发现提供了令人信服的证据,反对为这种分子电机使用英寸模型.
这项研究完善了我们对肌肉素V的过程性运动的理解.

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