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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
Published on: October 2, 2012
甲尼丁脱碳酶的活性对于细胞转化至关重要
M Auvinen1, A Paasinen, L C Andersson
1Department of Pathology, University of Helsinki, Finland.
Nature
|November 26, 1992
概括
甲尼丁脱碳酶 (ODC) 作为原瘤基因,驱动细胞转化和生长. 抑制ODC阻断瘤基因诱导的细胞转化,突出其在癌症发展中的核心作用.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 聚氨酸对细胞增殖至关重要,由鸟脱酶 (ODC) 调节.
- 在由致癌物,病毒或瘤基因诱导的细胞转化过程中,ODC表达升高.
研究的目的:
- 调查甲酸脱碳酶 (ODC) 是否调解细胞转化并具有致癌性质.
- 确定ODC在瘤基因诱导的细胞转化中的作用.
主要方法:
- 传染NIH3T3细胞与人类ODCcDNA在感觉和反感觉方向.
- 评估细胞转化,结独立生长和蛋白质氨酸酸化.
- 抑制内源性ODC并使用反意义RNA阻止v-src瘤基因的转化.
主要成果:
- 过度表达ODC诱导的细胞转化,固独立生长,以及130K蛋白的氨酸酸化.
- 反意义ODCRNA表达与表皮细胞形态和减少扩散相关.
- ODC抑制或反感性RNA合成阻止了v-src瘤基因诱导的纤维细胞转化.
结论:
- 甲素脱碳酶 (ODC) 基因作为原瘤基因起作用.
- ODC在调节细胞生长和转变方面发挥着关键作用.
- 准ODC可能为癌症治疗提供治疗策略.
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