性别依赖基因表达和德洛索菲拉转录组的演变
José M Ranz1, Cristian I Castillo-Davis, Colin D Meiklejohn
1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.
Science (New York, N.Y.)
|June 14, 2003
概括
取决于性别的选择驱动了Drosophila基因表达的快速进化. 大多数物种之间的基因表达差异都涉及性别偏差基因的变化,特别是在男性中.
科学领域:
- 进化遗传学的进化遗传学
- 进行比较的基因组学.
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 物种之间的基因表达差异可以揭示进化过程.
- 性偏差基因表达是常见的,并且迅速演变.
- 青虫黑虫和青虫模拟虫是比较研究的有价值的模型.
研究的目的:
- 研究Drosophila melanogaster和Drosophila simulans之间性别依赖基因调节的演变.
- 确定与性别偏差调节相关的基因表达分歧模式.
主要方法:
- 两个Drosophila物种的成年雄性和雌性之间的基因表达特征的比较分析.
- 消息传递 RNA 丰度差异的量化.
- 对性别偏见表达模式及其变化 (获利,损失,逆转) 的分析.
- 不同表达基因的功能聚类.
主要成果:
- 大约50%的基因显示了物种之间的表达差异.
- 其中,83%的人表现出改变的性别偏见表达 (获利,损失,增加,减少,逆转).
- 跨物种表达差异主要影响男性偏向基因.
- 只有表达不同的性别偏见基因才观察到功能聚类.
结论:
- 取决于性别的选择是Drosophila.快速基因表达进化的重要驱动因素.
- 性别偏差基因表达的变化在很大程度上导致了密切相关的物种之间的转录组的分歧.
- 德洛索菲拉转录组中的快速进化的基因被丰富为性别依赖的调节.
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