Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

High-resolution reconstruction of cell-type-specific transcriptional regulatory processes from bulk sequencing samples.

Nature biotechnology·2026
Same author

Dose-dependent hormone actions at estrogen receptor enhancers specify distinct molecular and biological outcomes.

Science advances·2026
Same author

Mapping the Subtype-Specific PARP1 ADP-ribosylated Proteome in Breast Cancer Cells.

Molecular cancer research : MCR·2026
Same author

PARP16 is a Druggable Regulator of Ribosome MARylation and Protein Homeostasis in Ovarian Cancer Cells.

bioRxiv : the preprint server for biology·2026
Same author

X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancer.

Oncogene·2026
Same author

Cooperative and competitive interactions among transcription regulatory elements modulate transcription output.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jul 14, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

在PARP的转录中,PARP进行了转录.

W Lee Kraus1, John T Lis

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

Cell
|June 18, 2003
PubMed
概括

在基因调节方面,PARP-1 (Poly(ADP-ribose) 聚合酶1 (PARP-1) 是至关重要的. 它修改了组织蛋白去解染色素,并在调节复合体中起作用,这两者都对体内转录至关重要.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 是一个遗传学.

背景情况:

  • 聚ADP-ribose) 聚合酶1 (PARP-1) 是一种参与DNA修复和基因表达的酶.
  • PARP-1催化了ADP-ribose单位转移到向蛋白质的过程.

研究的目的:

  • 阐明PARP-1在转录调节中的特定作用.
  • 了解PARP-1的酶活性如何影响染色体结构和基因表达.

主要方法:

  • 这项研究可能涉及生物化学测试,以评估PARP-1活性.
  • 可能使用了染色体免疫沉 (ChIP) 和基因表达分析.
  • 为了验证发现,进行了体内研究.

主要成果:

  • PARP-1 修改基因组,创建一个多基因基因组 (ADP-ribose) 矩阵,去凝结色素.
  • 这种脱凝使得DNA更容易被转录.
  • PARP-1还作为增强剂/促进剂监管复合物的组成部分.
  • 染色体修饰和调节复合体的作用都对生物体内基因调节至关重要.

结论:

更多相关视频

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter
07:53

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter

Published on: April 17, 2026

相关实验视频

Last Updated: Jul 14, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 2, 2010

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter
07:53

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter

Published on: April 17, 2026

  • PARP-1 在转录调节中起着双重作用:调节染色体结构和参与调节复合体.
  • 这些功能对于活体中适当的基因表达至关重要.