染色体凝聚力是由一个时钟基因类似的TIM-1调节的
Raymond C Chan1, Annette Chan, Mili Jeon
1Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3204, USA.
Nature
|June 27, 2003
概括
凯诺哈比迪斯的TIM-1通过与凝聚素复合体结合来调节染色体凝聚力. 破坏TIM-1或凝聚素会导致线粒和介质缺陷,显示出它在基因组稳定性中的保留作用.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 忠实基因组传输依赖于凝聚性,这是一个复制染色体连接复制染色体的复杂.
- 在染色体凝聚力中,TIMELESS类蛋白质的功能尚未完全理解.
研究的目的:
- 研究Caenorhabditis elegans TIM-1在染色体凝聚中的作用.
- 定义C. elegans凝聚复合体及其与TIM-1的相互作用.
主要方法:
- 生物化学实验来定义C. elegans的凝聚复合体.
- 在TIM-1或凝聚性破坏时评估线粒和介质染色体行为.
- 研究凝聚性子单位的组装.
主要成果:
- TIM-1在物理上与C. elegans凝聚复合体有关.
- 破坏TIM-1或凝聚素导致异常染色体行为和胚胎致死性.
- TIM-1耗尽影响非SMC凝聚性子单位组件,而SMC组件仍然负载.
- 同时耗尽TIM-1和SMC子单元会损害同源染色体配对.
结论:
- 在C. elegans.中,TIM-1在调节染色体凝聚力方面发挥着至关重要的作用.
- 凝聚素在甲基动物的同类染色体配对中发挥着新的作用.
- 染色体凝聚力,而不是生理时钟调节,很可能是TIMELESS类蛋白质的古老功能.
相关概念视频
Polytene Chromosomes
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also regularly...
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...


