在芽酵母中,Cks1-依赖的蛋白酶组招募和激活CDC20转录
May C Morris1, Peter Kaiser, Stanislav Rudyak
1The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Nature
|June 27, 2003
概括
通过调节Cdc20转录,Cks1蛋白在酵母中促进了线粒分裂. 它有助于解离Cdc28激酶,并将蛋白质组招募到CDC20促进体,以获得适当的基因表达.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- Cks 蛋白质是与循环林依赖激酶相互作用的保存蛋白质,但它们的功能仍然不清楚.
- 现有的遗传学,生物化学和结构生物学研究尚未为Cks蛋白功能提供统一的模型.
研究的目的:
- 研究Cks1在Saccharomyces cerevisiae中的重要作用.
- 阐明Cks1促进线粒分裂的机制.
主要方法:
- 研究了Cks1在Saccharomyces cerevisiae中的作用.
- 研究了Cks1与CDC20促进体,Cdc28激酶和蛋白质组的相互作用.
主要成果:
- Cks1对于促进线粒分裂至关重要.
- Cks1调节了Cdc20的转录激活,Cdc20是APC/C蛋白-ubiquitin结合酶的激活剂.
- 对于Cdc28激酶与CDC20促进体的周期性解离以及蛋白质组与促进体的周期性关联,Cks1是必需的.
结论:
- Cks1在线粒分裂中的重要作用包括调节Cdc20转录.
- Cks1可能通过招募蛋白质组和/或从CDC20促进体中解离Cdc28激酶来促进转录.
- 这种作用重塑了CDC20基因中的转录复合体或染色质,促进了细胞分裂.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...