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相关概念视频

Cross-reactivity00:42

Cross-reactivity

Overview
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...

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相关实验视频

Updated: Jun 25, 2026

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

抗体域交换是碳水化合物集群识别的免疫解决方案.

Daniel A Calarese1, Christopher N Scanlan, Michael B Zwick

  • 1Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Science (New York, N.Y.)
|June 28, 2003
PubMed
概括

人类抗体2G12通过结合病毒外上的保存糖来中和HIV-1. 这种抗体形成了一个独特的二元体,使其能够与人类免疫缺陷病毒 (HIV) 高亲和度结合.

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
09:39

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)

Published on: September 17, 2019

相关实验视频

Last Updated: Jun 25, 2026

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)
09:39

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics (DCAF)

Published on: September 17, 2019

科学领域:

  • 免疫学 免疫学 免疫学
  • 结构生物学 结构生物学
  • 病毒学 病毒学

背景情况:

  • 人类抗体2G12中和了各种人类免疫缺陷病毒1型 (HIV-1) 隔离物.
  • 它的目标是gp120信封糖蛋白"沉默"面上的密集的碳水化合物部分集群.

研究的目的:

  • 阐明2G12广泛中和的结构基础.
  • 了解高亲和度结合于保存的病毒表位的机制.

主要方法:

  • 对Fab 2G12及其复合物的X射线晶体学.
  • 生物化学,生物物理和突变发生分析.

主要成果:

  • 晶体结构显示,两个Fab组装成一个互锁的VH域交换二元体.
  • 这种Fab二元化形式是普遍存在的抗体配置,可以识别gp120.
  • 双相提供了一个扩展的表面,用于gp120上的多价值相互作用与保存的寡曼诺糖.

结论:

  • 抗体2G12独特的Fab-二度结构对其广泛的HIV-1中和至关重要.
  • 这种数字间域配置代表了碳水化合物表位的高亲和度识别的新机制.
  • 这一发现对开发针对病毒和微生物表面的碳水化合物向疗法有影响.