黑色素是盲鼠在非图像形成的光反应中所需的
Satchidananda Panda1, Ignacio Provencio, Daniel C Tu
1Genomics Institute of the Novartis Research Foundation, 10675 John J. Hopkins Drive, San Diego, CA 92121, USA.
概括
缺乏棒/光受体和黑色素的小鼠失去了所有的光反应,这表明视觉和非视觉系统对于哺乳动物的光感知至关重要.
科学领域:
- 神经科学是一个神经科学.
- 眼科医生 眼科 眼科
- 时间生物学 时间生物学
背景情况:
- 没有棒和光受体的小鼠是盲目的,但保留光介导反应.
- 表达黑色素的光敏感视网膜质细胞 (ipRGCs) 参与了这些非视觉反应.
- 棒和也可能有助于非视觉摄影.
研究的目的:
- 研究含有黑色素的ipRGCs和经典光受体 (棒和) 在哺乳动物的非视觉光反应中的作用.
主要方法:
- 使用了基因改造的小鼠,这些小鼠有外视网膜退化和黑色素缺乏症.
- 评估了昼夜振荡器的光训练.
- 测量了瞳孔对光的反应.
- 量化阿里胺-N-乙转移酶 (AA-NAT) 转录的光性抑制.
- 评估了光引起的运动活动的急性抑制.
主要成果:
- 缺乏外视网膜光受体和黑色素的小鼠完全缺乏光训练.
- 这些小鼠也没有表现出瞳孔光响应,没有AA-NAT的光抑制,也没有通过光激发运动活动的急性抑制.
- 这表明,用于调解这些非视觉反应,棒/圆光受体和黑色素都是必要的.
结论:
- 经典的视觉光受体 (棒和) 和基于黑色素的非视觉系统都对介导哺乳动物对光的各种非视觉反应至关重要.
- 这突出了不同光受体系统在调节昼夜节律和其他光驱动行为方面的综合功能.
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