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细胞关联和细胞外脂转移蛋白在人类冠状动脉动脉样硬化中

Kevin D O'Brien¹, Simona Vuletic, Thomas O McDonald

¹Division of Cardiology, Department of Medicine, University of Washington, Seattle, Wash, USA.

|July 2, 2003

概括

脂转移蛋白 (PLTP) 在人类动脉样硬化病变中被发现,这表明它在细胞胆固醇的积累和细胞外基质内脂蛋白的保留中发挥了作用. 这表明PLTP可能直接导致动脉样硬化的发展.

科学领域:

心血管生物学心血管生物学
动脉样硬化研究动脉样硬化研究
脂蛋白代谢脂蛋白的代谢

背景情况:

脂转移蛋白 (PLTP) 参与HDL代谢和肝脏VLDL分泌.
它在人类动脉样硬化病变形成中的直接作用尚不清楚.

研究的目的:

调查PLTP在人类动脉硬化冠状动脉病变中的存在和分布.
探索PLTP在细胞胆固醇代谢和这些病变中的细胞外矩阵相互作用中的潜在作用.

主要方法:

在人类冠状动脉中对PLTP,细胞类型 (SMC,巨细胞),阿波利波蛋白 (apoA-I,apoB,apoE) 和蛋白质糖的免疫组织化学分析.
在体外研究涉及胆固醇载荷的巨细胞,以评估PLTP的表达和活性.
凝移动性转移测试以评估PLTP与大甘和高密度脂结合的相互作用.

主要成果:

在正常动脉中,PLTP免疫染色是最小的,但在动脉样硬化病变中广泛存在,无论是细胞内还是细胞外.
在泡细胞SMC和巨细胞中检测到PLTP,胆固醇加载增加了PLTPmRNA,蛋白质和活性.
细胞外PLTP与apoA-I,apoB,apoE和biglycan结合在一起,增强HDL与biglycan的结合.

结论:

在人类动脉样硬化病变中存在PLTP.
PLTP分布表明它参与了细胞胆固醇代谢和脂蛋白在细胞外基质上的保留.

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