用紫外线圆形二重化谱学研究的水溶液中的三的稳定构造
Fatma Eker1, Kai Griebenow, Reinhard Schweitzer-Stenner
1Departments of Biology and Chemistry, University of Puerto Rico, Río Piedras Campus, P.O. Box 23346, San Juan, Puerto Rico 00931.
Journal of the American Chemical Society
|July 3, 2003
概括
像三氨酸一样,的精确溶液结构揭示了有序聚二和无序β-链形状之间的平衡. 这种结构受到溶剂同位素和键的影响,这表明随机线圈比以前认为的更有结构.
科学领域:
- 生物物理学的生物物理.
- 结构生物学 结构生物学
- 化学物理 化学物理
背景情况:
- 了解溶液中的和蛋白质结构对于蛋白质折叠和药物开发至关重要.
- 的"随机线圈"状态通常被认为是无结构的,但新出现的证据表明更大的内在秩序.
研究的目的:
- 使用紫外线圆二极化 (UVCD) 光谱测定三氨酸的溶液结构.
- 研究不同溶剂 (D2O,H2O,糖醇) 和同位素对形状的影响.
- 探索其他小的结构倾向,如三和三素.
主要方法:
- 紫外线循环二极化 (UVCD) 光谱法用于测量各种溶剂中的光谱.
- 进行了热力学分析,以量化稳定不同形状的能量贡献.
- 谱光学数据与振动谱光学和先前研究的预测进行了比较.
主要成果:
- 溶液中的三氨酸存在于聚烯II (3(1) - 螺旋和一个无序的β-链形状的混合物中.
- 热力学分析表明,聚二的平稳定,在室温下对β链的热偏好抵消.
- 观察到同位素效应,与D2O相比,H2O中的聚烯II占用率更高,突出显示了键的作用.
- 甘油可以稳定聚二烯II形状,类似于水.
- 三氨酸和三氨酸分别采用β-链和左转转结构.
结论:
- 的"随机线圈"状态比通常认为的结构更为显著,在螺旋和链形状之间具有平衡.
- 与溶剂分子的结合在稳定溶液中的二次结构方面发挥着关键作用.
- 和之间的微妙平衡影响了氨基酸残留物的结构偏好.
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