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相关概念视频

Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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相关实验视频

Updated: Jul 5, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

在光活性黄色蛋白中进行光异构和质子转移.

Michael J Thompson1, Donald Bashford, Louis Noodleman

  • 1Department of Molecular Biology, MB4, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.

Journal of the American Chemical Society
|July 3, 2003
PubMed
概括

计算研究揭示了对光活性黄色蛋白 (PYP) 光循环的新见解. 我们澄清了早期的中间体,并挑战了关于染色体质子动态的现有理论.

科学领域:

  • 生物物理学的生物物理.
  • 计算化学的计算化学
  • 频谱学是一种光谱学.

背景情况:

  • 光活性黄色蛋白 (PYP) 是一种细菌光传感器,采用了对库马里尔酸染色体.
  • PYP的光循环涉及由蓝光吸收引发的形状变化.
  • 之前的研究对早期光循环中间体和染色体质突产生不确定性.

研究的目的:

  • 通过计算来研究PYP光循环的早期中间体.
  • 解决结构数据和光谱测量之间的争议.
  • 阐明染色体光异构化和质子转移动态.

主要方法:

  • 使用了时间依赖密度函数理论 (TDDFT).
  • 计算包括光异构化和质子转移的能量概况.
  • 激发能被计算出来以识别光循环中间体.

主要成果:

  • 用于光异构化计算的潜在能量表面与实验光谱参数一致.
  • 计算的激发能量支持将一个冷捕获的中间体分配到PYP (B) (I) (0)).
  • 在不同温度下PYP (B) 光谱的差异是由染色体质子状态解释的.

更多相关视频

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

相关实验视频

Last Updated: Jul 5, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
05:54

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Published on: September 8, 2023

结论:

  • 在室温下,PYP(B) 中间体具有主要是无质子化的染色体,挑战了先前的信念.
  • 在PYP(L) 中介的染色体被计算确定为deprotonated,同意实验.
  • 在Glu46和染色体之间质子的移动性比以前认为的要高,影响了PYP的中间理解.