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相关概念视频

pre-mRNA Processing02:01

pre-mRNA Processing

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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相关实验视频

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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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酵母非编码RNA处理的全景图.

Wen Tao Peng1, Mark D Robinson, Sanie Mnaimneh

  • 1Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada.

Cell
|July 3, 2003
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概括
此摘要是机器生成的。

研究人员发现了许多新的酵母蛋白与核糖核蛋白 (RNP) 生物发生有关. 分析非编码RNA配置文件揭示了对RNA处理至关重要的未表征蛋白质,扩大了我们对RNP合成的理解.

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科学领域:

  • 分子生物学分子生物学
  • 酵母遗传学 酵母遗传学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • рибо核蛋白质 (RNP) 是必要的分子机器,涉及到许多细胞过程.
  • 参与RNP生物发生的蛋白质的完整补充,特别是在酵母中,仍然不完全表征.
  • 功能性基因组学和蛋白质组学数据为识别新组件提供了潜在的途径.

研究的目的:

  • 识别参与核糖核蛋白 (RNP) 生物发生的新型酵母蛋白.
  • 全球评估无特征酵母蛋白在非编码RNA处理中的作用.

主要方法:

  • 利用了公开可用的酵母功能基因组学和蛋白质组学数据的预测分析.
  • 采用微阵列来监测468种酵母突变菌株的非编码RNA丰度和处理.
  • 进行了进一步的实验,以验证已识别的因素在非编码RNA生物发生中的作用.

主要成果:

  • 预测分析表明,比目前已知的更多的蛋白质参与了RNP生物发生.
  • 对468种酵母菌株的分析揭示了非特征基因突变体中异常的非编码RNA配置文件.
  • 实验证实了参与非编码RNA生物发生的10个因素,包括影响rRNA前处理,核外体功能和snoRNA积累的蛋白质.

结论:

  • 目前大量未被描述的酵母蛋白在非编码RNA生物发生过程中发挥着关键作用.
  • 这项研究提供了酵母非编码RNA处理的整体观点,并确定了新的RNP生物发生因子.
  • 这些发现扩大了已知的参与酵母中RNP合成和加工的蛋白质景观.