相关实验视频
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Mechanical Manipulation of Neurons to Control Axonal Development
Published on: April 10, 2011
控制轴突分支动力学通过相关活动 in vivo 的控制
Edward S Ruthazer1, Colin J Akerman, Hollis T Cline
1Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA.
概括
视觉体验引导大脑连接的发展. 图案化的视觉活动促进了错误的轴突分支的消除,在光学纹状体中改进了地形图.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 视觉系统开发 视觉系统开发
背景情况:
- 轴突投射形成复杂的神经电路,由遗传和活动依赖的机制指导.
- 在光学纹路中开发拓地图对于视觉处理至关重要.
研究的目的:
- 调查视觉活动模式如何调节视网膜轴突突出的发育 in vivo.
- 了解视觉体验在细化轴突分支和地形地图形成中的作用.
主要方法:
- 在体内的视网膜轴突的时间延迟成像在白色Xenopus.
- 诱导双眼内置的光学带膜.
- 对N-甲基-D-酸盐 (NMDA) 受体的药理学阻断.
主要成果:
- 轴突在不同地区的分支尖端增加率相似.
- 轴突分支的选择性消除主要发生在被对侧眼主导的触摸区域.
- 阻断NMDA受体取消了这种经验依赖的分支消除.
结论:
- 视觉体验通过基于关联的机制直接影响轴突分支动态.
- 对于轴突突出的经验依赖的精细化,NMDA受体信号传递至关重要.
- 这个过程有助于在视觉系统中精确地形成地形图.
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