大肠直肠癌的幸存者和分子病原体
Paul J Kim1, Janet Plescia, Hans Clevers
1Department of Cancer Biology and the Cancer Center, University of Massachusetts, Medical School, Worcester, MA, USA.
Lancet (London, England)
|July 30, 2003
概括
转录因子T细胞因子 (TCF) /β-catenin激活了结直肠癌细胞中的幸存基因表达. 这可能会通过促进细胞生长和预防细胞死亡来驱动癌症.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 遗传学 是一个遗传学.
背景情况:
- 结肠直肠癌的发展与结肠密室细胞中的异常基因表达有关.
- T细胞因子 (TCF) /β-catenin通路与这种异常基因表达有关.
- 调节细胞死亡和增殖的蛋白质Survivin在结肠直肠癌中重新表达.
研究的目的:
- 研究TCF/β-catenin在调节结直肠癌中幸存者基因表达中的作用.
- 了解生存素在结直肠癌细胞中重新表达的机制.
主要方法:
- 促进体分析,突变发生和电泳性移动性转移试验被用于研究结直肠癌细胞中的基因表达.
- 在现场杂交和免疫组织化学检查了胚胎肠道中的生存表达.
- 在具有稳定β-catenin突变的细胞系中监测了亡变化.
主要成果:
- 在结直肠癌细胞中,TCF/β-catenin显著增加了幸存者基因表达 (6-12倍).
- 幸存者促进体中TCF结合元件的突变酶减少了75-79%的基因表达.
- 稳定β-catenin突变增强了幸存者的表达,并防止了细胞亡.
结论:
- 幸存者的TCF/β-catenin激活可能会给结肠密室赋予类似干细胞的表型.
- 这种表型涉及增加细胞增殖和对亡的抵抗力.
- 这些发现有助于理解结直肠癌的分子病原性.
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