相关实验视频
Updated: Jun 30, 2026

09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
通过比较基因组学识别的RNA编辑的神经系统目标
Barry Hoopengardner1, Tarun Bhalla, Cynthia Staber
1Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA.
概括
研究人员发现了一种新的方法,使用比较基因组学在基因中找到RNA编辑部位. 这导致识别了17个作用于RNA (ADARs) 的腺脱氨酶的新点,这对神经系统功能至关重要.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 神经科学是一个神经科学.
背景情况:
- 作用于RNA的腺氨酸脱氨酶 (ADARs) 催化RNA编辑,将信使RNA中的腺氨酸转化为 inosine.
- 在历史上,ADAR目标基因的发现是偶然的,限制了已知的RNA编辑范围.
- RNA编辑在遗传重编码中起着作用,但其全部范围和功能意义仍然不完全理解.
研究的目的:
- 开发一种系统的方法来识别作用于RNA (ADAR) 基因的新型腺脱氨酶.
- 通过识别新的ADAR目标,研究RNA编辑在神经系统中的作用.
- 描述新发现的RNA编辑站点的功能影响.
主要方法:
- 使用比较基因组学来识别表明RNA编辑的遗传学特征.
- 生物信息分析被用来预测跨物种潜在的ADAR基因.
- 进行了实验验证,以确认已识别的ADAR目标基因和编辑站点.
主要成果:
- 确定了RNA编辑的新型遗传特征,使得一种非偶然发现方法成为可能.
- 在Drosophila melanogaster中发现了16个以前未知的ADAR标基因,在人类中发现了1个.
- 新发现的ADAR标主要涉及快速电气和化学神经传递.
- 许多已识别的RNA编辑站点导致保存和功能关键氨基酸的重新编码.
结论:
- 比较基因组学为发现新型RNA编辑事件和目标提供了强大的工具.
- 通过ADARs调节的RNA编辑在神经系统功能中起着重要且以前被低估的作用.
- 通过RNA编辑对氨基酸的功能重新编码突出了其在神经元过程中的重要性.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

