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连续心脏磁共振成像注射的介质细胞干细胞

Jonathan M Hill¹, Alexander J Dick, Venkatesh K Raman

¹Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, Room 2c713, Bethesda, MD 20892-1538, USA.

|August 13, 2003

概括

铁化颗粒 (IFP) 标签允许使用MRI进行非侵入性跟踪中干细胞 (MSCs). 这种方法可以在跳动的心脏中检测移植的MSC,有助于干细胞研究.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
医疗成像医学成像

背景情况:

在跳动的心脏中的内肌心干细胞的非侵入性跟踪具有挑战性.
介质细胞干细胞 (MSC) 具有治疗潜力,但需要有效的输送和跟踪方法.

研究的目的:

开发和评估一种方法来标记MSCs与铁粒子 (IFP) 在体内MRI跟踪.
评估移植后IFP标记的MSC的即时和长期局部化.

主要方法:

从猪身上分离出MSC,并以剂量依赖的方式用IFP标记.
被标记的MSC被注射到正常和心脏梗塞的猪肌肉中.
连续心脏MRI (1.5T) 进行,以评估细胞随时间的局部化.

主要成果:

IFP标签是有效的,并没有影响MSC的生存能力,增殖或差异化.
核磁共振可以检测到只含有10^5 MSC的注射部位.
组织学证实了IFP携带的MSC在注射部位的存在.

结论:

对MSC的IFP标签提供了有价值的MRI对比,用于在活体中检测跳动的心脏.
该技术允许在注射部位使用MRI和光显微镜识别移植的MSC.
该技术有助于对干细胞保留,移植和迁移进行体内研究.

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...