实际上,FACT促进了转录依赖于核细胞的核细胞改变
Rimma Belotserkovskaya1, Sangtaek Oh, Vladimir A Bondarenko
1Howard Hughes Medical Institute, Department of Biochemistry, Division of Nucleic Acids Enzymology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA.
Science (New York, N.Y.)
|August 23, 2003
概括
该FACT复合物通过去除基因组二次体来促进RNA聚合酶II转录通过核体. 这种复合体还沉积着组织蛋白,在基因表达过程中保持染色质结构.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体动力学 染色体动力学
背景情况:
- 染色体,DNA和蛋白质的复合体,对转录构成了障碍.
- 众所周知,FACT (促进色素转录) 复合体对于RNA聚合酶II (Pol II) 的转录延长至关重要.
研究的目的:
- 阐明FACT复合物通过核细胞体促进转录的机制.
- 为了研究FACT复合体的组织伴侣活性.
主要方法:
- 在体外转录测试使用纯化的FACT复合物和核细胞DNA.
- 生物化学分析以评估基因素去除和沉积活动.
主要成果:
- 事实破坏了核细胞结构的稳定性,通过去除一个组素H2A-H2B二分体,使得Pol II通过.
- 事实上,FACT表现出内在的基因伴活动,能够将核心基因沉积到DNA上.
- FACT的活性取决于其子单元和Spt16.的酸性C端.
结论:
- 在转录过程中,FACT扮演着双重的角色:促进通过核细胞体的通行,并保持基因组的完整性.
- 该机制涉及动态核细胞体重塑和质子管理,保存表观遗传信息.
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