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追踪FACT和RNA聚合酶II延长复合体通过活体内染色体

Abbie Saunders¹, Janis Werner, Erik D Andrulis

¹Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA.

Science (New York, N.Y.)

|August 23, 2003

概括

该FACT蛋白质复合体通过促进核细胞分解来帮助RNA聚合酶II (Pol II) 转录. 在体内,FACT与活跃转录的Pol II基因结合,但不是Pol III基因,支持其在转录合核酶去除中的作用.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
染色体生物学染色体生物学

背景情况:

RNA聚合酶II (Pol II) 转录蛋白质编码基因,并且必须在核细胞中导航.
众所周知,FACT (促进染色体转录) 综合体在体外有助于Pol II转录.
在体内转录过程中核细胞管理的机制仍然不完全理解.

研究的目的:

在基因转录过程中调查FACT复合物的体内关联和功能.
确定FACT的定位是否与染色体上的特定RNA聚合酶活性相关.

主要方法:

在Drosophila聚烯染色体上FACT的免疫定位.
与Pol II和Pol III转录的基因进行FACT定位的比较.
在体内分析FACT招募和跟踪动力学.

主要成果:

在活体中,FACT被招募到并跟踪活跃转录的Pol II基因.
在生物体内,FACT的动力学与Pol II和延长因子Spt5和Spt6.6的动力学相似.
实际上,FACT不会与Pol III转录的基因共局.

结论:

实际上,FACT与Drosophila染色体上的Pol II转录有关.
这些发现表明FACT在涉及核细胞分解的转录过程中的功能.
FACT的选择性局部化支持其在Pol II转录期间克服核细胞障碍的作用.

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