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相关概念视频

Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Catalysis02:50

Catalysis

The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes a mild...
Catalysis01:27

Catalysis

Catalysis influences the rate of chemical reactions by providing an alternative reaction pathway with lower activation energy. A catalyst speeds up a reaction, but it is not consumed during the process. The fundamental principle of catalysis is the ability of a catalyst to alter the reaction mechanism, often introducing a more efficient pathway than the uncatalyzed process.In a catalyzed reaction, the catalyst participates directly in the reaction mechanism. It interacts with reactants to form...

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相关实验视频

Updated: Jul 7, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

一个关于酶催化剂的观点.

Stephen J Benkovic1, Sharon Hammes-Schiffer

  • 1Department of Chemistry, 152 Davey Laboratory, Pennsylvania State University, University Park, PA 16802, USA. sjb1@psu.edu

Science (New York, N.Y.)
|August 30, 2003
PubMed
概括

这项研究检查了酶催化假设,强调了蛋白质分子运动,特别是二叶酸还原酶,对于催化效率至关重要. 保存的残留物网络影响酶结构和运动,影响进化和蛋白质工程.

科学领域:

  • 生物化学 生物化学
  • 理论化学 理论化学
  • 结构生物学 结构生物学

背景情况:

  • 酶催化是生物过程的基础.
  • 许多假设被提出来解释酶机制.
  • 蛋白质动态在酶功能的作用仍然是一个活跃的研究领域.

研究的目的:

  • 仔细检查酶催化剂的开创性假设.
  • 研究蛋白质分子运动对催化性能的影响.
  • 探索酶动态的进化和工程影响.

主要方法:

  • 对酶催化物的生物化学和理论观点的历史综述.
  • 分析蛋白质内的分子运动.
  • 使用二叶酸减少酶的案例研究.

主要成果:

  • 酶的催化效率受到内部分子运动的显著影响.
  • 保存残留物的结合网络决定了蛋白质的结构和动态.
  • 二叶酸还原酶作为一个模型,展示了这些合网络.

结论:

  • 蛋白质分子运动是酶催化过程中不可或缺的一部分.

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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

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Assaying Protein Kinase Activity with Radiolabeled ATP
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Assaying Protein Kinase Activity with Radiolabeled ATP

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相关实验视频

Last Updated: Jul 7, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
11:16

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Published on: July 11, 2012

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

  • 保存的残留网络在酶的进化和功能中发挥着关键作用.
  • 了解这些网络为先进的蛋白质工程提供了潜力.