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通过一种细菌的III型效应器裂开阿拉比多普西斯PBS1.

Feng Shao¹, Catherine Golstein, Jules Ade

¹Department of Biological Chemistry, Medical School and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.

Science (New York, N.Y.)

|August 30, 2003

概括

植物疾病耐药性蛋白质间接检测病原体的毒性蛋白质. Pseudomonas注射器中毒性蛋白 AvrPphB 通过分裂 PBS1 蛋白激酶来激活耐药性,通过酶活性来检测.

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科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

植物疾病抗性 (R) 蛋白通常识别病原体抗性 (Avr) 蛋白.
大多数Avr蛋白的精确生化功能及其R蛋白激活机制在很大程度上是未知的.
RPS5和PBS1对于阿拉比多普西斯对表达AvrPphB.B.的Pseudomonas注射器菌株的耐药性至关重要.

研究的目的:

为了阐明 AvrPphB 激活 RPS5 中介植物免疫的机制.
为了确定AvrPphB是否直接或间接触发了植物的抗药反应.

主要方法:

研究了Arabidopsis.在AvrPphB和PBS1之间的相互作用.
使用生物化学试验分析了PBS1.1上的AvrPphB的活性.
评估了对于RPS5介导的疾病耐药性的PBS1裂变的要求.

主要成果:

AvrPphB被确定为一种蛋白酶,它专门分裂PBS1蛋白激酶.
发现,AvrPphB对PBS1的裂变对于RPS5依赖性耐药性至关重要.
这表明植物检测到AvrPphB的酶活性,而不是蛋白质本身.

结论:

植物R蛋白可以通过它们的酶活性间接检测病原体毒性因素.
AvrPphB采用一种蛋白质分解机制,通过PBS1和RPS5.5激活植物免疫信号.
这项研究揭示了在植物防御中识别病原体效应者的新模式.

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