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相关概念视频

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

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相关实验视频

Updated: May 12, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

随着MPF的振荡,cdc25和cdc2-cyclin B之间有周期性关联.

C Jessus1, D Beach

  • 1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724.

Cell
|January 24, 1992
PubMed
概括

仙人掌卵细胞含有p72,一个cdc25的亲属,激活cdc2.2. 在M阶段,p72与cdc2-cyclin B结合,这表明细胞周期控制涉及蛋白相互作用,而不仅仅是基因调节.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 发展生物学 发展生物学

背景情况:

  • 线粒分裂的开始需要cdc2蛋白激酶的激活.
  • 成熟促进因子的激活取决于cdc2氨酸脱化.
  • cdc25是特定的氨酸酸酶,可以激活cdc2.

研究的目的:

  • 为了研究cdc25亲属p72在Xenopus卵细胞和胚胎中的作用.
  • 为了确定p72是否在细胞周期期间与cdc2-cyclin B相互作用.
  • 探索超越转录调节的线粒控制机制.

主要方法:

  • 在Xenopus卵细胞中识别p72.
  • 在Xenopus胚胎的细胞周期期间分析p72的丰度.
  • 评估p72与cdc2-cyclin B的相关性.
  • 检测M相相关激酶的催化活性.

主要成果:

  • 奇诺普斯卵细胞具有相对的cdc25,p72.
  • 在Xenopus胚胎的细胞周期中,p72的丰度不会波动.
  • p72与cdc2-cyclin B表现出细胞周期依赖的关联,在M阶段达到峰值.

更多相关视频

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

相关实验视频

Last Updated: May 12, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

  • 与p72相关的M相激酶具有催化活性.

    • 结论:

    • 甲基控制可能涉及cdc25和环林B-cdc2复合体之间的周期性物理关联.
    • 除了对cdc25基因的转录调节之外,还可以通过其他机制来影响线粒控制.