c-Jun和MyoD蛋白之间的功能对抗:直接的物理关联
E Bengal1, L Ransone, R Scharfmann
1Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92186-5800.
Cell
|February 7, 1992
概括
原生原蛋白 Jun 蛋白质通过干扰 MyoD 蛋白质来抑制肌肉发育. MyoD和Jun在物理上相互作用,影响肌肉发育中的基因调节.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 瘤基因是一种瘤基因.
背景情况:
- 原型瘤基因 Jun 是一种转录因子,参与各种细胞过程.
- 肌源性差异化1 (MyoD) 是调节肌肉发育的关键转录因子.
- 在肌肉发育过程中Jun和MyoD之间的相互作用尚未完全理解.
研究的目的:
- 为了研究Jun在肌肉发育中的抑制作用.
- 阐明Jun和MyoD之间的相互作用背后的分子机制.
- 确定Jun如何影响MyoD功能和基因表达.
主要方法:
- 暂时转染试验用于评估促进剂和增强剂的活性.
- 对与MyoD和AP-1结合部位相关的记者基因表达的分析.
- 在体内和体外共免疫沉以检测蛋白质与蛋白质相互作用.
- 对Jun和MyoD相互作用域的突变分析.
主要成果:
- Jun的构成表达通过干扰MyoD来抑制肌形成.
- Jun抑制了MyoD调控的促进剂和增强剂的交换活化.
- MyoD对抗了AP-1位点链接基因的Jun介导的交换活化.
- 在体内和体外证实了MyoD和Jun蛋白之间的物理相互作用.
- 这种相互作用涉及Jun的leucine拉链域和MyoD的螺旋环-螺旋环区域.
结论:
- 原型瘤基因Jun通过与MyoD蛋白质的直接物理相互作用来抑制肌体发生.
- 这种相互作用调节了这两种蛋白质的转录活性,影响了肌肉细胞的分化.
- 了解Jun-MyoD相互作用,可以了解肌肉发育的调节和潜在的瘤性途径.
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