Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
ATP Synthase: Structure01:18

ATP Synthase: Structure

ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Structure of Amines01:19

Structure of Amines

The hybridized nitrogen atom in amines possesses a lone pair of electrons and is bound to three substituents with a bond angle of around 108°, which is less than the tetrahedral angle of 109.5°. However, the C–N–H bond angle is slightly larger at 112°, with a carbon–nitrogen bond length of 147 pm. This carbon–nitrogen bond length of of amines is longer than the carbon–oxygen bond of alcohols (143 pm) but shorter than alkanes’ carbon–carbon bond (154 pm). These aspects are illustrated in Figure...
Unit Cells01:18

Unit Cells

A crystal's internal structure is an orderly array of atoms, ions, or molecules, and the details of this array significantly influence the solid's properties. In a crystal, periodically repeating 'structural motifs' - which could be atoms, molecules, or groups thereof - create a 'space lattice.' This is essentially a three-dimensional, infinite array of points, each surrounded by its neighbors in an identical way, forming the basic structure of the crystal.A 'unit cell' is a theoretical...
Imperfections in Crystal Structure: Point, Line and Plane Defects01:25

Imperfections in Crystal Structure: Point, Line and Plane Defects

A perfect crystal, in theory, has a uniform structure with the same unit cell and lattice points throughout. However, any deviation from this periodic arrangement is known as an imperfection or defect. These defects can be categorized into three types: point, line, and plane defects.Point defects occur when there is a deviation from the ideal due to missing atoms, displaced atoms, or additional atoms. These imperfections might occur due to imperfect packing during crystallization or because of...
Imperfections in Crystal Structure: Stoichiometric Point Defects01:26

Imperfections in Crystal Structure: Stoichiometric Point Defects

Schottky defects arise when some lattice points in a crystal, such as those in NaCl, remain unoccupied, creating lattice vacancies without disturbing the overall electrical neutrality of the crystal. This defect is common in ionic crystals where the positive and negative ions are similar in size, as seen in sodium chloride and cesium chloride. The presence of Schottky defects enables the crystal to conduct electricity to a small extent through an ionic mechanism. Electric fields cause nearby...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An evaluation of the implementation of a parent-led, games-based physical activity intervention: the Active Play at Home quasi-randomized trial.

Health education research·2018
Same author

Correction: Analysis of unstable modes distinguishes mathematical models of flagellar motion.

Journal of the Royal Society, Interface·2017
Same author

Early-life glucocorticoids programme behaviour and metabolism in adulthood in zebrafish.

The Journal of endocrinology·2016
Same author

Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart.

Molecular and cellular endocrinology·2015
Same author

Analysis of unstable modes distinguishes mathematical models of flagellar motion.

Journal of the Royal Society, Interface·2015
Same author

Physiological roles of glucocorticoids during early embryonic development of the zebrafish (Danio rerio).

The Journal of physiology·2013

相关实验视频

Updated: Jul 16, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

一个dUTPase的晶体结构.

E S Cedergren-Zeppezauer1, G Larsson, P O Nyman

  • 1Department of Zoological Cell Biology, Wenner-Gren Institute, University of Stockholm, Sweden.

Nature
|February 20, 1992
PubMed
概括

大肠杆菌的dUTPase可以阻止 uracil 融入DNA. X射线结晶学揭示了它的3D结构,显示了一个具有果卷折叠的三元酶和复杂的子单元相互作用,这对催化有潜在的关键.

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 酶学 是一种酶学.

背景情况:

  • dUTPase酶通过水解dUTP来防止乌拉加入DNA.
  • 大肠杆菌dUTPase具有严格的基质特异性,通过糖和来区分核酸.
  • 维持低细胞内dUTP水平对于DNA完整性至关重要.

研究的目的:

  • 为了确定大肠杆菌dUTPase的三维结构.
  • 阐明dUTPase的基质特异性的结构基础.
  • 研究基于结构特征的潜在催化机制.

主要方法:

  • 使用X射线结晶学来确定酶的结构.
  • 高分辨率数据收集时间为1.9年.
  • 对三级和四级结构的分析.

主要成果:

  • 已经解决了大肠杆菌dUTPase的三维结构.
  • 酶形成了一个对称的三元体.
  • 三级结构的特点是糖果卷折叠,与经典的核酸结合域不同.
  • 在四级结构中观察到复杂的子单元相互作用.
  • 保存的序列元素位于这些相互作用的附近.

更多相关视频

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

相关实验视频

Last Updated: Jul 16, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

结论:

  • 确定的结构为大肠杆菌dUTPase的分子结构提供了洞察力.
  • 独特的结构特征,包括子单元相互作用,可能在催化中发挥作用.
  • 进一步的研究可以探索这些结构性发现的功能影响.