在S相期间cdk2激酶与转录因子E2F的结合
M Pagano1, G Draetta, P Jansen-Dürr
1European Molecular Biology Laboratory, Heidelberg, Germany.
Science (New York, N.Y.)
|February 28, 1992
概括
转录因子E2F在细胞周期的S阶段与环林A和cdk2蛋白激酶相互作用. 这种相互作用需要一个活跃的cdk2激酶,这表明它在调节细胞增殖中的作用.
科学领域:
- 分子生物学分子生物学
- 细胞周期调节细胞周期调节
- 瘤发生的发生因子.
背景情况:
- 转录因子E2F调节细胞增殖所必需的基因.
- E2F是已知的腺病毒E1A瘤基因的目标.
- 细胞循环进展涉及复杂的调节网络,包括蛋白激酶和环林.
研究的目的:
- 研究人类细胞中E2F,环林A和cdk2蛋白激酶之间的相互作用.
- 为了确定cdk2与E2F的细胞周期特定结合.
- 阐明cdk2激酶活性在E2F复合体形成中的作用.
主要方法:
- 同免疫沉测试检测蛋白质复合体.
- 西方涂抹分析蛋白质表达和细胞周期阶段.
- 激酶测试以评估cdk2活动.
主要成果:
- 在人类细胞中,发现了循环A和cdk2蛋白激酶与E2F的复合体.
- 特别是在细胞周期的S阶段观察到Cdk2与E2F的结合.
- 总cdk2水平在整个细胞周期中保持不变,但其与E2F的结合取决于细胞周期.
结论:
- cdk2和E2F之间的相互作用是由细胞周期调节的.
- 对于E2F结合,需要一个活跃的cdk2激酶,可能与环林A复合.
- 这些发现表明,在细胞增殖过程中,E2F介导的基因表达受到控制的机制存在.
相关概念视频
01:45Positive Regulator Molecules
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02:39Positive Regulator Molecules
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02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
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Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.