拉斯对于神经生长因子和醇诱导的MAP激酶的铁酸酸化是必不可少的
S M Thomas1, M DeMarco, G D'Arcangelo
1Howard Hughes Medical Institute, Department of Microbiology, University of Pennsylvania, Philadelphia 19104.
Cell
|March 20, 1992
概括
在PC12细胞中,Ras信号传递对神经生长因子 (NGF) 诱导的氨酸酸化中至关重要. 这种途径的激活发生在Ras作用之前和之后.
科学领域:
- 细胞信号通道是细胞信号通道.
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 神经生长因子 (NGF) 对PC12细胞的处理触发了快速的蛋白质氨酸酸化.
- 在NGF诱导的细胞反应中Ras信号的作用需要进一步阐明.
研究的目的:
- 调查Ras在NGF和TPA诱导的氨酸酸化中的作用.
- 为了确定Ras在响应NGF和TPA刺激时调节的特定蛋白质.
主要方法:
- PC12细胞被感染了主要的抑制性或瘤性Ras突变.
- 评估了细胞蛋白的氨酸酸化水平.
- 免疫沉和西式涂抹被用来识别脂蛋白.
- 线素激活蛋白激酶 (MAPK) 活性通过体外激酶试验测量.
主要成果:
- 拉斯信号特别阻断了NGF和TPA诱导的42和44kDa蛋白的氨酸酸化.
- 这些相同蛋白质的瘤性Ras诱导的氨酸酸化的表达.
- 44kDa的蛋白质被确定为细胞外信号调节激酶1/MAPK,而42kDa的蛋白质与MAPK结合.
- 拉斯调节的MAPK激活,但不是NGF诱导的Trk激活或PLC-gamma 1酸化.
结论:
- 拉斯在NGF和TPA诱导的MAPKs的铁酸化中发挥着关键作用.
- NGF诱导的氨酸酸化发生在Ras信号的上游和下游.
- MAPKs是PC12细胞中对NGF和TPA的反应中Ras的关键下游作用因子.
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