ras通过三种信号转导蛋白激酶的神经生长因子受体调节进行介导:MAP激酶,Raf-1和RSK
K W Wood1, C Sarnecki, T M Roberts
1Program in Cell and Developmental Biology, Harvard University, Boston, Massachusetts.
Cell
|March 20, 1992
概括
拉斯蛋白 (p21c-ras) 对于细胞生长至关重要,但它们的信号通路尚不清楚. 这项研究表明,ras介导神经生长因子和蛋白激酶C对MAP激酶,RSK和Raf-1的影响.
科学领域:
- 蜂信号传输是如何进行的
- 分子生物学分子生物学
- 瘤发生的发生因子.
背景情况:
- p21c-ras对于氨酸激酶刺激的细胞生长和分化至关重要.
- p21c-ras使用的特定信号通路在很大程度上是未知的.
- 了解这些途径是解读细胞对生长因子反应的关键.
研究的目的:
- 为了阐明p21c-ras.ras.传播的下游信号通路.
- 研究ras在调节MAP激酶,RSK和Raf-1中的作用.
- 为了确定生长因子和博 Ester 如何通过 ras.激活这些信号分子.
主要方法:
- 在PC12细胞中利用了ras (c-Ha-ras(Asn-17) 的主要抑制突变.
- 评估了MAP激酶 (ERK家族),RSK和Raf-1的激活.
- 比较表达主导抑制突变细胞与表达激活ras瘤基因的细胞中的信号.
主要成果:
- 表达c-Ha-ras ((Asn-17) 抑制生长因子和醇诱导的MAP激酶,RSK和Raf-1激酶的激活.
- 相反,激活的ras瘤基因的表达足以刺激这些信号事件.
- 这些发现确定了ras作为这些信号级联中的中心调解者.
结论:
- 拉斯蛋白是神经生长因子受体和蛋白质激酶C的信号传递的关键媒介.
- 拉斯影响MAP激酶,RSK和Raf-1的激活.
- 这项研究提供了一个更清晰的了解的ras-mediated细胞生长和分化途径.
相关概念视频
mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...


