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一个基于异常稳定的2',5'-链接r (UUCG) 循环结构的新型RNA基因

Alexei Yu Denisov¹, Rami N Hannoush, Kalle Gehring

¹Department of Biochemistry and Montreal Joint Centre for Structural Biology, McGill University, Montreal, QC, H3G 1Y6, Canada.

Journal of the American Chemical Society

|September 18, 2003

概括

2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 4 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 4 4 4 1

科学领域:

分子生物学分子生物学
生物化学生物化学
结构生物学结构生物学

背景情况:

有3',5'-连接环的原生RNA发针在热力学上是稳定的.
2',5'-链接RNA环表现出优越的稳定性,独立于干的组成.

研究的目的:

确定具有2',5'-链接RNA (UUCG) 循环的稳定发针的溶液结构.
研究2',5'-RNA环的增强稳定性的结构基础.

主要方法:

核磁共振 (NMR) 光谱法用于确定溶液结构.
基配对,基堆叠和糖与糖的相互作用的分析.

主要成果:

2',5'-链接RNA循环采用了与3',5'-链接循环不同的一种新折叠.
稳定性归因于U5:G8的摇摆基配对,广泛的基层堆叠以及糖基/糖糖接触.
U5:G8基对延伸了茎,而U6堆叠在U5和C7上,暴露了.

结论:

2',5'-链接的核糖核酸的独特结构和结合解释了这些四环的增强稳定性.
2',5'-RNA循环代表了一种新的结构图案,对设计基于RNA的疗法有意义.
这些发现支持在阿普坦, рибо酶和反感应剂中使用2',5'-RNA循环.

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