人类催产素受体的结构和表达
1Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Nature
|April 9, 1992
概括
研究人员确定了人类催产素受体的结构和表达. 肌膜中催产素受体信使RNA的增加有助于在分娩期间提高敏感性.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 与G蛋白结合的受体是G蛋白结合的受体.
背景情况:
- 在分娩前,子宫肌膜对催产素的敏感性大大增加.
- 这种高度敏感性与催产素受体的显著增加有关.
- 了解催产素受体的结构和表达对于理解分娩开始至关重要.
研究的目的:
- 确定人类催产素受体互补DNA (cDNA) 的结构和表达.
- 为了研究催产素受体的功能反应.
- 为了阐明在分娩期间肌肉细胞中催产素受体水平增加的基础分子机制.
主要方法:
- 表达式克隆用于隔离人类催产素受体cDNA.
- 分析了受体的结构,揭示了典型的G蛋白结合受体特征.
- 功能性研究涉及表达Xenopus卵细胞中的受体,以评估催产素反应.
- 在各种人体组织中分析了信使RNA (mRNA) 的大小和水平.
主要成果:
- 人类的催产素受体是一种388氨基酸多,具有7个跨膜域.
- 在Xenopus卵细胞中表达的催产素受体特别对催产素作出反应,诱导膜电流.
- 在不同组织中检测到两种大小的催产素受体mRNA (3.6kb和4.4kb).
- 在术后的子宫肌膜中观察到4.4kbmRNA的非常高水平.
结论:
- 已确定的人类催产素受体结构与G蛋白结合受体一致.
- 功能表达证实了氧化通过其受体的特定作用.
- 产后肌胎性催产素受体mRNA的大量增加在分娩期间观察到的受体数量的增加中起作用.
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