德罗斯菲拉超螺旋通过异构体形成调节了ecdysone受体的功能
T P Yao1, W A Segraves, A E Oro
1Howard Hughes Medical Institute, La Jolla, California.
Cell
|October 2, 1992
概括
草超 (usp),一个RXR同类,与ecdysone受体 (EcR) 合作调节基因表达. 这一发现突显了受体异体化在内分泌系统中的古老作用.
科学领域:
- 分子内分泌学分子内分泌学
- 进行比较基因组学.
- 昆虫生理学 昆虫生理学
背景情况:
- 脊椎动物的视网状X受体 (RXRs) 通过异构体复合体调节激素信号传递.
- 超螺旋 (usp) 是RXR.的Drosophila同类物.
研究的目的:
- 研究Drosophila ultraspiracle (usp) 在核受体信号传递中的功能作用.
- 为了确定drosophila usp的合作伙伴,并阐明它们的功能相互作用.
主要方法:
- 在刺激DNA结合时,对RXR进行USP的功能替代试验.
- 鉴定了ecdysone受体 (EcR) 作为 usp 的结合伙伴.
- 在培养的哺乳动物细胞中进行共传染实验,以评估ecdysone的反应性.
主要成果:
- 虫可以替代脊椎动物的RXR,激活各种核受体的DNA结合.
- usp与ecdysone受体 (EcR) 形成了一个高度合作的DNA结合复合体.
- 在哺乳动物细胞中,EcR和usp的同时表达对于ecdysone诱导的基因表达至关重要.
结论:
- 草是功能性埃克迪松受体 (EcR) 综合体的一个组成部分.
- 涉及RXR/usp的受体异构化可能早于脊椎动物和无脊椎动物的血统分歧.
- 这突显了RXR和usp在核受体介导的内分泌调节和进化中的保留,基本作用.
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