疫苗和牛病毒编码了一种新型分泌的介质素-1-结合蛋白
M K Spriggs1, D E Hruby, C R Maliszewski
1Department of Molecular Biology, Immunex Research and Development Corporation Seattle, Washington 98101.
Cell
|October 2, 1992
概括
疫苗病毒产生一种蛋白质,该蛋白质结合interleukin-1β,抑制淋巴细胞的增殖,并可能导致毒性. 破坏这种病毒基因可以减少这些影响.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 病毒,包括疫苗病毒 (VV),编码调节宿主免疫反应的蛋白质.
- 介素-1β (IL-1β) 是炎症和免疫细胞激活中的关键细胞因子.
研究的目的:
- 为了识别和表征一种与小鼠IL-1β (mIL-1β) 结合的疫苗病毒蛋白.
- 研究这种病毒IL-1β结合蛋白在病毒毒性和免疫调节中的作用.
主要方法:
- 对IL-1β结合的病毒开放读取框架 (ORF) 的表达和特征.
- 构建和分析具有破坏IL-1β结合蛋白基因 (vB15RKO) 的复合疫苗病毒.
- 对IL-1β结合,淋巴细胞增殖抑制和小鼠病毒毒性的评估进行了测试.
主要成果:
- 从感染VV的细胞中鉴定出一种33kDa的蛋白质和一种同类于IL-1受体的VVORF,它们特别结合mIL-1β.
- 来自牛病毒的类似ORF也与mIL-1β结合.
- 在vB15RKO中VV ORF的破坏消除了超水生物中的IL-1β结合蛋白.
- 来自VV感染细胞的超级活体抑制了IL-1诱导的淋巴细胞增殖,而来自vB15RKO感染细胞的超级活体没有.
- 被破坏的ORF (vB15RKO) 表明在VV病毒性中发挥了作用.
结论:
- 疫苗病毒编码了一种IL-1β结合蛋白,可以干扰宿主免疫反应.
- 这种病毒蛋白可能在毒杆病毒感染的发病和毒性中发挥重要作用.
- 了解这些病毒性免疫调节器对于理解波克斯病毒与宿主相互作用至关重要.
相关概念视频
Viral Recombination
22.3K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
22.3K
Leaky Scanning
4.5K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
4.5K
The JAK-STAT Signaling Pathway
10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.2K
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Immune Response Against Viral Pathogens
2.4K
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
2.4K
Inhibitors of Viral Protein Synthesis
73
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
73


