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Updated: Jun 21, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
14:44

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Published on: December 16, 2013

使用二氧化复合物作为一个多功能1H NMR识别探头

Z W Li1, H Taube

  • 1Department of Chemistry, Stanford University, CA 94305.

Science (New York, N.Y.)
|April 10, 1992
PubMed
概括

一个新的基探头[en2Os(eta2-H2) ]2+,通过独特的NMR信号识别出各种生物分子. 这个探测器可以区分微妙的结构差异在分子,如核酸和氨基酸.

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¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

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¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
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2D NMR: Overview of Heteronuclear Correlation Techniques

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