在哺乳动物神经元中,ATP调解了快速的突触传输
R J Evans1, V Derkach, A Surprenant
1Vollum Institute, Oregon Health Sciences University, Portland 97201.
Nature
|June 11, 1992
概括
腺三酸盐 (ATP) 作为快速激发性神经递质,在腹腔中起作用. 研究人员通过观察其对突触潜力和电流的影响来证实ATP的作用,而这与其他神经递质不同.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 神经传递的神经传递
背景情况:
- 腺三酸盐 (ATP) 已知具有细胞内功能,但也与细胞表面受体相互作用.
- P2X-纯诺受体是细胞表面受体,被认为可以作为连接体带离子通道发挥作用.
- P2X受体存在于各种神经元上,表明ATP作为快速激发性神经递质的潜在作用.
研究的目的:
- 为了研究ATP作为神经递质在培养的性质神经元中的神经神经元突触中的作用.
- 为了确定ATP是否在这个特定的神经元路径中模仿内源性神经传递.
主要方法:
- 从培养的性质神经元中记录了刺激性突触潜力和电流.
- 管理ATP并观察其对突触活动的影响.
- 利用特定的抗剂 (suramin) 和激动剂 (alpha,beta-methylene-ATP) 来表征所涉及的受体.
- 测试了对其他神经递质受体 (尼古丁性,血清性,葡萄糖性,GABAergic,上腺性,腺能性) 抗剂的作用.
主要成果:
- 应用ATP模仿了观察到的激发性突触潜力和电流.
- ATP的作用被P2-纯素受体对手suramin阻断.
- 观察到阿尔法,β-甲基-ATP,一个已知的P2X受体激活剂的脱敏.
- 其他主要神经递质受体的对手不影响突触活动,排除了他们的参与.
结论:
- 在研究中,ATP 作为内源性神经递质,在胆固醇中的神经神经元突触中起作用.
- 这一发现确定了纯能神经递质在自主性质中发挥的特定作用.
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