相关实验视频
Updated: Jun 21, 2026

13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
抑制平滑肌肉细胞增殖的反感性寡氧核化物,向编码增殖细胞核抗原的信使RNA
1Cardiology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
Circulation
|August 1, 1992
概括
针对增殖细胞核抗原 (PCNA) 的反感性寡氧核化物 (ODN) 有效抑制了血管光滑肌肉细胞的增殖. 这种方法在冠状动脉血管塑性手术后有望预防静脉缩.
科学领域:
- 血管生物学 血管生物学
- 分子医学是分子医学.
- 基因规则 基因规则
背景情况:
- 血管光滑肌细胞 (SMC) 增殖是冠状动脉血管塑性手术后复原的一个关键因素.
- 目前的策略集中在抑制细胞表面生长因子信号传递上.
- 这项研究探讨了一种针对细胞内通路的替代方法.
研究的目的:
- 为了研究反感性寡度氧核化物 (ODN) 抑制增殖细胞核抗原 (PCNA) 表达的疗效.
- 为了确定抑制PCNA是否会减少SMC的扩散.
主要方法:
- 小鼠大动脉SMCs被修改的反感性ODNs (15或18-mer) 治疗.
- 度在10至100微米之间.
- 用西方斑点和免疫细胞化学评估PCNA表达;通过细胞计数测量细胞增殖.
主要成果:
- 这种18-mer反意义的ODN显著降低了SMC的增长超过50%,以一种依赖于度的方式.
- 这种对增殖的抑制作用持续了至少9天.
- 西方斑块和免疫细胞化学证实PCNA蛋白表达减少.
结论:
- 针对PCNA mRNA的反意义ODN有效降低PCNA基因产物表达.
- 这种PCNA的减少导致SMC增殖的减少.
- 这些发现支持进一步的体内调查,以使用反感应策略预防冠状动脉缩.
相关概念视频
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Transducer Mechanism: Nuclear Receptors
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

