四个homeobox基因的嵌套表达域在发育的面向大脑中的表达域
A Simeone1, D Acampora, M Gulisano
1International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Nature
|August 20, 1992
概括
研究小鼠头部发育,研究人员确定了四个关键基因:Emx1,Emx2,Otx1和Otx2. 它们的表达模式揭示了早期大脑区域化至关重要的嵌套领域.
科学领域:
- 发育生物学是发展生物学.
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
背景情况:
- 了解脊椎动物的头部发育是有限的,特别是与干部发育相比.
- 像空螺旋体和正牙体这样的多索菲拉基因对头部发育至关重要.
- 鼠标Emx1和Emx2基因与空螺旋相关,在发育中的前脑中表达.
研究的目的:
- 为了识别和描述与Drosophila orthodenticle相关的小鼠基因.
- 为了比较发育中的小鼠大脑中Emx1,Emx2,Otx1和Otx2的表达域.
- 阐明这些基因在前脑区域特征中的作用.
主要方法:
- 在小鼠中克隆和描述Otx1和Otx2基因.
- 在性交后第10天使用小鼠胚胎对基因表达模式的比较分析.
- 在发育中的面向大脑中绘制Emx1,Emx2,Otx1和Otx2的表达域.
主要成果:
- Otx2在大脑中广泛表达,分脑和大脑中脑.
- Otx1 表达式嵌套在 Otx2 域内.
- 埃姆克斯2和埃姆克斯1在背部脑部和脑区内呈现逐渐受限的表达域.
结论:
- 这四个基因 (Emx1,Emx2,Otx1,Otx2) 在发育中的小鼠大脑中表现出嵌套的表达模式.
- 这些嵌套域在发育早期建立,与大脑主要区域的区域规范相吻合.
- 这些发现为脊椎动物前脑模式的遗传控制提供了洞察力.
相关概念视频
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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...


