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相关概念视频

Recombinant DNA01:09

Recombinant DNA

Overview
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Recombinant DNA01:09

Recombinant DNA

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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相关实验视频

Updated: Jun 19, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

V(D) J重组:破碎的DNA分子与共价密封 (发针) 编码结束在scid小鼠胸细胞中.

D B Roth1, J P Menetski, P B Nakajima

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Cell
|September 18, 1992
PubMed
概括

严重联合免疫缺陷 (SCID) 鼠标淋巴细胞启动V(D) J重组,但难以连接DNA段. 与正常小鼠不同,SCID胸细胞显示了破损的DNA,其编码末端与发针密封,这表明SCID因子在V(D) J修复中发挥了作用.

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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

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Last Updated: Jun 19, 2026

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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • V(D) J重组对于适应性免疫受体基因组合至关重要.
  • 严重联合免疫缺陷 (SCID) 的特点是V(D) J重组的缺陷.
  • 之前的研究表明,SCID小鼠在V(D) J重组过程中DNA连接受损.

研究的目的:

  • 为了研究SCID小鼠淋巴细胞中V(D) J重组的特定缺陷.
  • 为了分析SCID胸细胞中TCR三角位点DNA断裂的性质.
  • 探索SCID因子在V(D) J重组机制中的潜在作用.

主要方法:

  • 从SCID和正常小鼠中分析淋巴细胞和胸细胞.
  • 在T细胞受体 (TCR) 三角位点检查DNA分子.
  • 研究DNA末端处理和连接机制.

主要成果:

  • SCID淋巴细胞启动V(D) J重组,但显示严重减少的编码分段连接.
  • 与正常小鼠不同的是,SCID胸细胞在TCR三角位点具有编码末端的破碎DNA.
  • 在SCID中,编码端被密封在发针结构中,而信号端没有.

结论:

  • 编码末端的发针形成可能是V(D) J重组的一个普遍的早期步骤.
  • 这种针头形成可以解释P核酸在编码关节中的起源.
  • 这些发现表明SCID因子在DNA裂变和V(D) J重组忠实性中的潜在作用.