预防红细胞度中的Yersinia enterocolitica生长
R N Pietersz1, H W Reesink, W Pauw
1Red Cross Blood Bank, Amsterdam, The Netherlands.
Lancet (London, England)
|September 26, 1992
概括
在处理和去除白细胞之前,将全血保持在22°C,可以显著减少Yersinia enterocolitica在血液制品中的生长. 这种方法提高了储存的红细胞的安全性.
科学领域:
- 微生物学 微生物学
- 输血医学 输血医学
- 食品安全 食品安全
背景情况:
- 叶氏菌 (Yersinia enterocolitica) 在血液制品中构成污染风险.
- 目前的加工方法可能无法完全缓解细菌生长.
- 确保血液产品的安全性对于患者健康至关重要.
研究的目的:
- 评估温度和白细胞减少对全血中Yersinia enterocolitica生长的影响.
- 确定最佳的加工步骤,以尽量减少红细胞缩中的细菌污染.
- 为了提高血液制品对Yersinia enterocolitica的安全性.
主要方法:
- 整体血液被接种了Yersinia enterocolitica. 这是一个非常好的方法.
- 血液成分在不同的温度条件下 (在20小时内4°C和22°C) 被加工成红细胞缩物 (RBC),缓冲外套耗尽的红细胞 (BCd-RBC) 和白细胞耗尽的红细胞 (Ld-RBC).
- 在4°C保存5周后,评估了细菌生长情况.
主要成果:
- 在短时间冷藏后处理的红细胞显示了Yersinia enterocolitica的最多生长.
- BCd-RBC的生长比红细胞的生长少.
- Ld-RBC显示细菌生长最少,表明显著减少.
- 在加工前将全血保持在22°C,可以减少细菌的增殖.
结论:
- 在22°C的温度下保持全血,可以利用固有的杀菌活性.
- 随后的白细胞去除进一步减少了Yersinia enterocolitica的污染.
- 推的加工包括初始加热至22°C,然后减少白细胞以获得更安全的血液制品.
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