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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 19, 2014
在转基因烟草中操纵花朵结构
M A Mandel1, J L Bowman, S A Kempin
1Department of Biology, University of California, San Diego, La Jolla 92093.
Cell
|October 2, 1992
概括
烟草花中AGAMOUS基因的异胎表达诱导了同源性转变,支持了花发育的遗传模型. 这表明了可预测的花朵结构操纵和基因功能跨物种的互换性.
科学领域:
- 植物遗传学 植物遗传学
- 发育生物学是发展生物学.
- 花的器官生成 植物的器官生成
背景情况:
- 花的发育是由同源基因 (A,B,C功能) 控制的.
- 这些基因在花原始体中指定了器官身份.
- 了解它们的相互作用是植物发育的关键.
研究的目的:
- 为了测试花同源功能的遗传模型.
- 为了研究AGAMOUS基因 (C功能) 的作用.
- 为了评估物种之间的基因功能互换性.
主要方法:
- 生成的转基因烟草植物.
- 在异位表达的Brassica napus AGAMOUS基因.
- 观察到由此产生的花表型.
主要成果:
- 转基因的花朵表现出同源的转变:分叶变成,花变成茎.
- 观察到的表型与遗传模型的预测相匹配.
- 宫外AGAMOUS表达证实了C功能的充分性.
结论:
- 通过改变单个调节性基因表达,可以预先操纵花结构.
- 的基因功能是保存和远距离相关物种之间可互换的.
- 这项研究验证了花器官特异性的遗传模型.
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