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Antigenic Liposomes for Generation of Disease-specific Antibodies
Published on: October 25, 2018
高亲缘性IgE受体的激活激活了与SRC蛋白相关的氨酸激酶
1Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical, Research Institute, Princeton, New Jersey 08543-4000.
Nature
|January 2, 1992
概括
高亲和性IgE受体 (Fc epsilon RI) 通过激活与SRC蛋白相关的不同类型的氨酸蛋白激酶 (TPKs),如p56lyn和p62c-yes,这些激酶与受体结合,从而触发过敏反应.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 高亲和性IgE受体 (Fc epsilon RI) 对于即时的过敏反应至关重要.
- 在巨细胞和基细胞上Fc epsilon RI的参与启动了信号级联,包括蛋白质氨酸酸化.
- 虽然Fc epsilon RI子单元缺乏激酶活性,但相关的激酶酸化受体组件.
研究的目的:
- 为了确定在不同细胞模型中在Fc epsilon RI交联时激活的特定铁蛋白激酶 (TPKs).
- 调查激活TPK与Fc epsilon RI复合体之间的关联.
- 阐明SRC蛋白相关TPKs在Fcepsilon RI介导的信号传导中的作用.
主要方法:
- 使用大鼠基性白血病 (RBL-2H3) 和小鼠巨细胞 (PT-18) 线.
- 使用Fc epsilon RI交叉连接来触发细胞反应.
- 执行共同免疫沉测试以检测蛋白质相互作用.
- 通过生物化学测试评估氨酸蛋白激酶激活.
主要成果:
- 在RBL-2H3细胞中,Fc epsilon RI交联激活了p56lyn和pp60c-src,p56lyn与受体共同免疫.
- 在 PT-18 细胞中,Fc epsilon RI 接触激活了 p62c-yes,这是唯一可检测的 src 蛋白相关TPK,它也与受体共免疫.
- 这些发现表明,与SRC蛋白相关的特异TPKs可以与Fcepsilon RI结合,并在受体接触时被激活.
结论:
- 不同的src蛋白相关的铁蛋白激酶可以与高亲和性IgE受体 (Fc epsilon RI) 相结合.
- 这些相关TPK的激活是Fc epsilon RI交联后的一个关键事件.
- 这突出了Fc epsilon RI信号传递的保存机制,涉及不同细胞类型的不同SRC家族激酶.
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