对于L-选择因的内皮连接体是一种新型的粘素样分子
L A Lasky1, M S Singer, D Dowbenko
1Department of Immunobiology, Genentech, Inc,, South San Francisco, California 94080.
Cell
|June 12, 1992
概括
研究人员在淋巴结中发现了一种新型的素样型葡萄糖蛋白,它作为支架起作用. 这种分子向L-选择素呈现碳水化合物,调解淋巴细胞对高内皮静脉 (HEV) 的粘附,以进行免疫监测.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 淋巴细胞指向淋巴结的指导对于免疫反应至关重要.
- L-选择素调解了淋巴细胞对高内皮静脉 (HEV) 的粘附.
- 一种硫酸糖蛋白 (Sgp50) 之前被确定为L-selectin的HEV配体.
研究的目的:
- 为了净化Sgp50糖蛋白.
- 为了克隆编码Sgp50.50的cDNA.
- 描述Sgp50在淋巴细胞与HEV相互作用中的结构和功能.
主要方法:
- 净化Sgp50糖蛋白的净化方法.
- N-终端氨基酸测序. 氨基酸测序. 在 N-终端氨基酸测序.
- cDNA克隆和序列分析.
- 进行mRNA表达分析.
- 抗体的产生和免疫沉.
- 淋巴结HEVs的免疫组织化学染色.
主要成果:
- Sgp50的净化和cDNA克隆成功进行.
- 预测的蛋白质序列揭示了一种具有氨酸/氨酸丰富域的新型粘素样分子.
- Sgp50 mRNA优先表达在淋巴结中.
- 对Sgp50的抗体免疫降低了糖蛋白和染色的HEV表面.
- Sgp50作为一个支架,向L-selectin提供碳水化合物.
结论:
- 一种新的,特定于组织的粘素类内皮质糖蛋白 (Sgp50) 已被确定.
- 这种糖蛋白在调解淋巴细胞粘附于淋巴结HEV中起着关键作用.
- Sgp50可能通过向L-selectin lectin域呈现碳水化合物联体来起作用.
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