新的人类基因编码HIV Tat介导的转换激活的积极调节器
H Shibuya1, K Irie, J Ninomiya-Tsuji
1Institute for Molecular and Cellular Biology, Osaka University, Japan.
Nature
|June 25, 1992
概括
人类免疫缺陷病毒-1 (HIV-1) 蛋白 Tat 激活病毒基因表达. 一个新发现的细胞因子MSS1增强了Tat介导的交换激活,这表明它在HIV基因激活中起着关键作用.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 基因法规 基因法规
背景情况:
- 人类免疫缺陷病毒-1 (HIV-1) 蛋白 Tat 是病毒基因表达的一个关键的交换激活器.
- 在HIV-1长端重复中,Tat与TAR序列相互作用.
- 已知细胞因子与Tat合作,调节病毒基因表达.
研究的目的:
- 为了识别和描述参与Tat介导的HIV-1交换活化的细胞因素.
- 研究新发现的MSS1基因在HIV-1基因调节中的作用.
主要方法:
- 通过转补酵母sgv1-突变的转补MSS1的人类补充DNA (cDNA) 的分离.
- 序列同质分析将MSS1与已知的蛋白质进行比较,包括TBP-1.
- 内源MSS1信使RNA (mRNA) 水平与HIV-1激活之间的相关性分析.
- 实验评估MSS1对Tat中介交易激活的影响.
主要成果:
- 一个新的人类基因MSS1被分离出来,它可能编码了一个与Tat.合作的细胞因子.
- MSS1蛋白与已知的Tat相互作用蛋白TBP-1具有显著的序列相同性.
- 内源MSS1mRNA水平与Tat.的HIV-1激活程度具有积极的相关性.
- 表明MSS1的表达增强了HIV基因的Tat介导的交换活化.
结论:
- MSS1是一种细胞因子,在增强HIV-1的Tat介导转换活化方面发挥着重要作用.
- 这些发现表明MSS1是控制HIV基因表达的调控网络的关键组成部分.
更多相关视频
07:18High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
Published on: January 22, 2019
13:47Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
Published on: March 29, 2019
相关概念视频
Viral Mutations
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
LTR Retrotransposons
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
Size and Structure of Viral Genomes
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
Inhibitors of Virion Maturation and Assembly
As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
