Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Protein Modifications in the RER01:26

Protein Modifications in the RER

Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dishevelled C-terminus: prolyl and histidinyl motifs.

Acta physiologica (Oxford, England)·2011
Same author

Integration of transmembrane signaling Cross-talk among G-protein-linked receptors and other signal transduction pathways.

Trends in cardiovascular medicine·2011
Same author

Socioeconomic differences in risk factors for obesity in adolescents in Northern Ireland.

International journal of pediatric obesity : IJPO : an official journal of the International Association for the Study of Obesity·2007
Same author

Wheezing in Chinese schoolchildren: disease severity distribution and management practices, a community-based study in Hong Kong and Guangzhou.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2005
Same author

Ten-year trends for fatness in Northern Irish adolescents: the Young Hearts Projects--repeat cross-sectional study.

International journal of obesity (2005)·2005
Same author

PDE6 is an effector for the Wnt/Ca2+/cGMP-signalling pathway in development.

Biochemical Society transactions·2004

相关实验视频

Updated: Jul 12, 2026

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

对GS蛋白α子单元序列的反感性寡氧核酸加快了纤维细胞分化到脂肪细胞的过程.

H Y Wang1, D C Watkins, C C Malbon

  • 1Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China.

Nature
|July 23, 1992
PubMed
概括

霍乱毒素通过激活Gsαα,阻断3T3-L1纤维细胞分化成脂肪细胞. 针对Gs-alpha的反感性寡氧核酸加速了这种差异化,揭示了Gs-alpha独立于循环AMP的新角色.

科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 分子生物学分子生物学

背景情况:

  • 3T3-L1纤维细胞分化为脂肪细胞,积累脂质.
  • G蛋白对于跨膜信号传递至关重要,并且可以自我调节.
  • 在3T3-L1脂肪生成过程中,G蛋白水平发生显著变化.

研究的目的:

  • 研究G蛋白,特别是Gsα在3T3-L1纤维细胞分化中的作用.
  • 确定Gsα活性是否影响脂肪生成的速度和过程.

主要方法:

  • 用霍乱毒素对3T3-L1细胞进行治疗,以激活Gs alpha.
  • 使用针对Gs alpha的反感性寡氧核酸的药物.
  • 监测脂质积累和分化的时间进程.

主要成果:

  • 霍乱毒素抑制了脂肪细胞的分化.
  • 反理性Gsα oligodeoxynucleotides显著加速了分化,将时间从7-10天缩短到大约3天.
  • 百日咳毒素,福斯科林或迪布蒂瑞尔cAMP没有影响分化,这表明Gsα的作用独立于cAMP.

结论:

更多相关视频

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
11:59

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
06:53

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

相关实验视频

Last Updated: Jul 12, 2026

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
11:59

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
06:53

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

Published on: June 2, 2022

  • Gsα活性在调节3T3-L1脂肪生成中发挥着关键的新型作用.
  • 这种Gsα的调制是独立于细胞内循环AMP水平的变化.